Survey of Theileria annulata and Theileria buffeli/orientalis Complex in Cattle in the Kırşehir Region Using Multiplex-PCR
نویسندگان
چکیده
منابع مشابه
Detection of Theileria annulata carrier cattle by PCR.
A simple method for treating bovine blood samples for direct detection of T. annulata in carriers, after polymerase chain reaction (PCR) amplification of small subunit ribosomal RNA (SSU rRNA) gene is described. The threshold of detection of the PCR-assay was an erythrocytic parasitaemia of 0.00008% corresponding to 16 infected bovine erythrocytes. In 50 known carriers, 42 were positive in PCR,...
متن کاملDetection and Discrimination of Theileria annulata and Theileria lestoquardi by using a Single PCR
The aim of this study was to detect and differentiate Theileria annulata and T. lestoquardi (hirci) by PCR. Members of the genus Theileria are tick-borne hemoprotozoan parasites those cause fatal and enervating diseases of cattle and sheep in Iran . In order to develop a specific method for detecting and identification of Theileria species, specific primers from the surface protein (SP) seque...
متن کاملDevelopment of a multiplex PCR assay for simultaneous detection of Theileria annulata, Babesia bovis and Anaplasma marginale in cattle
Tropical theileriosis, bovine babesiosis and anaplasmosis are tick-borne protozoan diseases that impose serious constraints on the health and productivity of domestic cattle in tropical and sub-tropical regions of the world. A common feature of these diseases is that, following recovery from primary infection, animals become persistent carriers of the pathogen and continue to play a critical ro...
متن کاملBiochemical, Hematological studies in cattle naturally infected with Theileria annulata
The aim of the present study dealt with biochemical and hematological changes in cattle which presented to the Azad clinic of veterinary school in Urmia during the period between Dec 2008 to January 2012. A total of 985 cattle with positive theileriosis were examined. Data were collected on hematological conditions of the cattle. Ages of these slaughtered buffaloes ranged from 6 to 9 years, (79...
متن کاملDetection of Theileria annulata in blood samples of carrier cattle by PCR.
We report the detection of Theileria annulata, the causative agent of tropical theileriosis, by PCR in blood samples obtained from carrier cattle. The assay employs primers specific for the gene encoding the 30-kDa major merozoite surface antigen of T. annulata. A 721-bp fragment was amplified from blood samples taken monthly from calves experimentally infected with one of four different stocks...
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ژورنال
عنوان ژورنال: Turkish Journal of Parasitology
سال: 2012
ISSN: 1300-6320,2146-3077
DOI: 10.5152/tpd.20112.03